Application

Suggested dilution for most applications:

1:50,000-1:1,000,000 for ELISA using enzyme-conjugated streptavidin

1:10,000-1:100,000 for western blotting with chemiluminescent substrates.

1:500-1:5,000 for enzyme immunoohistochemistry on tissue sections.

1:200-1:1,000 for flow cytometry and fluorescence immunohisto /cytochemistry

Optimal working dilutions must be determined by the end user.

This Goat anti-Rabbit IgG Antibody, biotin-SP conjugate, Species Adsorbed is validated for use in ELISA, WB for the detection of Rabbit IgG.

Research CategorySecondary & Control Antibodies

Research Sub CategorySecondary Antibodies Adsorbed for Dual Labeling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Lyophilized. Buffer = 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, 0.01% Thimerosal and 0.05% Sodium Azide

RECONSTITUTION:

Reconstitute with 1.5 mL sterile distilled water.

Specificity

Rabbit IgG (H+L) (absorbed for minimal cross-reactivity to Human, Mouse and Rat Serum Proteins)

Storage and Stability

Maintain lyophilized product at 2-8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C with or without aliquotting for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol.